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RIP3对BCG感染后小鼠巨噬细胞凋亡的调控作用研究
受体相互作用蛋白3 卡介苗 RAW264.7细胞 细胞凋亡
2020/9/17
探讨受体相互作用蛋白3（RIP3）在卡介苗（BCG）诱导小鼠巨噬细胞RAW264.7凋亡过程中的调控作用。方法：构建RIP3腺病毒干扰载体并感染巨噬细胞，并用BCG进行感染。采用噻唑蓝（MTT）比色法检测细胞活力；利用流式细胞术对细胞凋亡率、线粒体膜电位及活性氧含量进行检测；通过Western blot检测RIP3及凋亡相关蛋白的表达水平。
细粒棘球绦虫重组BCG-EgG1Y162菌株的构建和表达
细粒棘球绦虫 EgG1Y162 重组卡介苗
2013/6/3
构建和表达细粒棘球绦虫重组卡介苗（BCG）菌株rBCG-EgG1Y162。 方法 通过基因工程技术将细粒棘球绦虫抗原EgG1Y162的编码基因与大肠埃希菌（E. coli）-分枝杆菌穿梭表达质粒载体pMV361重组，并转化E. coli后进行扩增。重组质粒pMV-EgG1Y162经PCR和双酶切鉴定后，进行测序。将鉴定正确的rpMV-EgG1Y162通过电穿孔技术转化至感受态BCG菌株中，构建...
研究幽门螺杆菌(Hp)不同表达量硫氧还蛋白1(Trx1)对人胃癌细胞(BCG-823)生长的作用。方法 将高表达Trx1及低表达Trx1 Hp分别以1∶50,1∶100,1∶200细胞细菌浓度比进行细菌细胞共培养，设不加菌空白对照组，分别于24 h及48 h后分别用倒置显微镜及MTT法观察细胞形态及细胞存活率,并收集1∶100浓度组细胞用流式细胞仪检测细胞周期。结果 从镜下观察及测细胞OD值均发现...
Post BCG Lymphadenitis in Vaccinated Infants in Yazd,Iran
Lymphadenitis BCG Vaccination Immunization Tuberculosis Infants
2009/12/15
Objective: Bacille Calmette-Guَerin (BCG) vacination is performed as a part of expanded program of immunization (EPI). Lymphadenitis is the most common complication of BCG vaccination. The aim o...
抑制NO诱生对BCG免疫性肝损伤中CYP1A2表达的影响
NO诱生 BCG免疫性肝损伤 CYP1A2表达
2009/7/28
目的: 研究卡介苗(BCG)所致小鼠免疫性肝损伤中，一氧化氮(NO)诱生对肝脏细胞色素P450药物代谢酶系CYP1A2亚型表达的影响.方法: 采用尾静脉注射BCG诱发小鼠产生免疫性肝损伤，HE染色法观察肝脏病理组织学变化，采用免疫组化法测定肝组织诱导型一氧化氮合酶(iNOS)及其CYP1A2的蛋白表达，采用图像梯度灰度扫描法对肝脏病理损伤与iNOS形成进行半定量相关性分析.结果: 尾静脉注射BCG...
Generalized BCG Lymphadenitis in an Infant:Diagnostic Dilemma With Lepromatous Leprosy and Gaucher Disease on Fine Needle Aspiration
MUSTAFA HACIMUSTAFAOĞ LU MEHMET OKAN Ö MER YERCİ SUNA GEDİ KOĞ LU İ RFAN KIRIŞ TIOĞ LU İ BRAHİ M ILDIRIM
2009/7/2
A two-year old male infant with generalized BCG lymphadenitis is presented. There was no obvious immunodeficiency disorder detected in the patient. BCG vaccine had been given at two months of age, and...
恶性疟原虫FCC1/HN株环子孢子蛋白基因在BCG中的表达。
目的 比较弓形虫致密颗粒蛋白4（GRA4）基因和表面抗原2（SAG2）基因的重组卡介苗（BCG）对小鼠的免疫保护效果。 方法 108只SPF级雌性BALB/c小鼠随机分成6组：PBS组、BCG空白菌组、BCG?鄄空白载体组、BCG?鄄SAG2组、BCG-GRA4组和BCG-SAG2+GRA4组，每组18只。每鼠分别注射对应液体/疫苗100 μl，共2次，间隔2周。接种前尾静脉采血，接种后4、6、8...
目的 探讨细粒棘球绦虫（Eg）重组BCG-Eg95疫苗免疫后对受攻击小鼠的包囊减重率及脾细胞淋巴因子的影响。方法 细粒棘球绦虫重组BCG-Eg95疫苗分别采用皮下注射、鼻腔内接种、口服灌胃和肌肉注射4种途径免疫BALB/C小鼠，免疫后8周用Eg原头节攻击感染，50个Eg原头节/每只小鼠，感染后18周剖杀小鼠，分离并称重细粒棘球蚴，计算包囊减重率；取脾，分离脾细胞，用Eg粗抗原（EgAg）或伴刀...
The Use of BCG（Bacille Calmette Guérin）as an Antispermatogenic Agent: Structural and Hormonal Changes
antispermatogenic agent BCG contraception orchitis granuloma
2009/2/19
The effectiveness of intratesticularly injected bacille Calmette Guérin (BCG) as an antispermatogenic agent was examined by light microscopy in rats after three, six, ten, twenty, and 56 days and in...
目的： 动态观察多房棘球绦虫重组BCG-Em14-3-3疫苗免疫小鼠后脾细胞因子的变化。方法： 将疫苗采用鼻腔内接种免疫BALB/C鼠，在免疫后0、2、4、6、8、10、12、14、16和18w各剖杀4只小鼠取脾，分离脾细胞，用EmAg或ConA刺激培养，收集脾细胞培养上清液，用试剂盒检测脾细胞培养上清液的IL-2、IFN-γ、TNF-α和IL-4水平，同时设有PBS对照。结果： 疫苗接种组的IL...
Serum Levels of anti-BCG, Albumin and Packed Cell Volume and White Blood Cell Count in Subjects with HIV and Malaria Co-morbidity
Malaria HIV⁄ AIDS BCG PCV Albumin
2010/12/10
The present study was designed to determine anti-BCG titres, serum albumin, packed cell volume, white blood cell count and malaria parasite density in HIV infected subjects in a malaria endemic area w...
PRESUMED SYSTEMIC BACILLE CALMETTE-GUÉRIN DISEASE AFTER BCG VACCINATION:REPORT OF A CLINICAL CASE
Disseminated BCG infection BCGosis
2010/4/6
BCG (bacille Calmette–Guérin) vaccine is administered worldwide to prevent severe forms
of tuberculosis. It is considered to be safe; however, occasional complications are seen. The most
serious com...
Disseminated Mycobacterium bovis Infection after BCG Vaccination
BCG Children Tuberculosis Vaccination
2010/2/1
The Calmette-Güerin vaccine (BCG) is administered to all the newborns in Iran in order to prevent tuberculosis. Complications of this vaccine are uncommon. We report disseminated BCG disease in 17 pat...
A Study of Relation between BCG Scar and Atopy in Schoolchildren of Zanjan City
Allergy Asthma BCG vaccine Dermatitis Atopic
2010/2/8
The prevalence of atopic disease in recent decades has been dramatically increased. It has been suggested that BCG vaccination may protect against development of allergic diseases. The purpose of this...