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Flow cytometric DNA analysis of defined stages of rat seminiferous epithelial cycle during in vitro differentiation
Flow cytometric DNA analysis rat seminiferous epithelial cycle
2009/2/23
The stages of the rat seminiferous epithelial cycle have been isolated for flow cytometric analysis of DNA and for culture, using transillumination-assisted microdissection. Precise stages have been...
Evaluation of oxidative DNA damage in human sperm and its association with male infertility
8-Hydroxydeoxyguanosine spermatozoa infertility
2009/2/18
Recently, there is increasing evidence suggesting that oxidative sperm DNA damage is closely associated with impaired sperm function and male infertility. 8-Hydroxydeoxyguanosine (8-OHdG) is consi...
Rat seminal-vesicle secretory protein SVS II binds DNA with a preference for the 5' regulatory region of secretory protein SVS IV gene：co-isolation with components of the nuclear matrix
caltrin nuclear scaffold nuclear structure semen tissue
2009/2/18
In rats, the ventral prostate and seminal vesicles produce distinct sets of proteins whose functions and tissue-specific regulation by androgens remain unclear. We have utilized the genes encoding t...
Enhanced susceptibility of follicle-stimulating-hormone-deprived infertile bonnet monkey（Macaca radiata）spermatozoa to dithiothreitol-induced DNA decondensation in situ
Enhanced susceptibility follicle-stimulating-hormone-deprived infertile bonnet monkey dithiothreitol-induced DNA decondensation
2009/2/17
Immunoneutralization of endogenous follicle-stimulating hormone (FSH) of adult male monkeys leads to oligospermia and infertility despite unchanged testosterone levels. The inability of these monkey...
Effect of semen dilution on bovine sperm viability as determined by dual-DNA staining and flow cytometry
Sperm concentration cellular viability SYBR-14
2009/2/17
Living and dead spermatozoa were examined for the effects of sperm concentration level on sperm viability. Semen was collected from two different bulls on each of four collection dates. A ninth bull...
Mammalian sperm DNA susceptibility to in situ denaturation associated with the presence of DNA strand breaks as measured by the terminal deoxynucleotidyl transferase assay
Acrldine orange chromatin structure SCSA TdTA
2009/2/13
Sperm from four mammalian species were analyzed by the sperm chromatin structure assay (SCSA) and the terminal deoxynucleotidyl transferase assay (TdTA) using flow cytometry. The SCSA quantitates the ...
Dual DNA staining assessment of bovine sperm viability using SYBR-14 and propidium iodide
Sperm fluorescence SYBR-14 flow cytometry vital staining cryopreservation
2009/2/13
A new membrane-permeant DNA stain, SYBR-14, was used in combination with propidium iodide (PI) to estimate the proportion of living sperm in bovine semen. The SYBR-14 stained living sperm while PI...
Previous studies from this laboratory on hamster spermatozoa have demonstrated that rodent sperm DNA is packaged into the sperm nucleus in a specific manner by nuclear structures. The entire genom...
4-MAPC, a 5 alpha-reductase inhibitor, reduces rat ventral prostate weight, DNA, and prostatein concentrations
4-MAPC, a 5 alpha-reductase inhibitor rat ventral prostate weight DNA prostatein concentrations
2009/2/7
Several compounds, such as 4-MAPC (4-methyl-3-oxo-4-aza-5 alpha-pregnane-20- carboxylate), that inhibit conversion of testosterone (T) to dihydrotestosterone (DHT) by 5 alpha-reductase have been demon...
DNA-flow cytometry of defined stages of rat seminiferous epithelium: effects of 3 Gy of high-energy X-irradiation
testis Sertoli germ cell
2009/2/6
Testes of adult Sprague-Dawley rats were irradiated locally by 3 Gy of 4 MeV X-rays produced by a linear accelerator. This type and dose of radiation gives an even distribution through the testis an...
Cryopreservation-Thawing of fractionated human spermatozoa is associated with membrane phosphatidylserine externalization and not DNA fragmentation
Phosphatidylserine translocation TUNEL annexin V
2008/12/29
The objective of these studies was to evaluate the effect of cryopreservation-thawing of human spermatozoa on DNA fragmentation and membrane integrity. This was a prospective, controlled cohort stud...
Functional and ultrastructural features of DNA-fragmented human sperm
DNA-fragmented human sperm
2008/12/25
The functional significance of deoxyribonucleic acid (DNA) fragmentation in ejaculated human sperm is unclear. In this study the extent of DNA strand breakage in swim-up selected spermatozoa was eva...
An immunochemical assay to detect DNA damage in bovine sperm
immunochemical assay DNA damage bovine sperm
2008/12/25
An immunochemical assay has been developed to detect oxidative damage in bovine sperm DNA. Sperm DNA contains a large amount of oxidative damage as a result of exposure to exogenous agents, but dama...
This review outlines the role of mitochondria in providing
energy for cell function and discusses the relevance of
mutations to various cell types within the mitochondrion’s
own genome. In particul...
DNA integrity in human spermatozoa：relationships with semen quality
DNA integrity human spermatozoa relationships semen quality
2008/12/25
The literature contains conflicting evidence regarding the existence of DNA damage in spermatozoa from infertile male patients. To examine this phenomenon, we have studied ejaculated spermatozoa fro...