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Two-dimensional gel electrophoretic analysis of rat sperm membrane interaction with cauda epididymal fluid
Two-dimensional gel electrophoretic analysis rat sperm membrane interaction cauda epididymal fluid
2009/2/23
Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) was used to analyze the polypeptide composition of rat cauda epididymal fluid, blood serum and membrane-enriched fractions of caput, corp...
Quantification of bovine sperm separation by a swim-up method. Relationship to sperm motility, integrity of acrosomes, sperm migration in polyacrylamide gel and fertility
Quantification bovine sperm separation swim-up method
2009/2/23
The number of bovine spermatozoa separated in a swim-up procedure was quantified using an electronic cell counter. In an initial test of the swim-up procedure, non-frozen sperm samples with differen...
Regional differences in luminal fluid polypeptides of the rat testis and epididymis revealed by two-dimensional gel electrophoresis
Regional differences luminal fluid polypeptides rat testis epididymis revealed two-dimensional gel electrophoresis
2009/2/20
Luminal fluid samples were collected by micropuncture of the seminiferous tubule, rete testis, and defined levels of the epididymal tubule. After removal of spermatozoa by centrifugation, the supernat...
Detection of DNA damage in response to cooling injury in equine spermatozoa using single-cell gel electrophoresis
DNA damage equine spermatozoa single-cell gel electrophoresis
2008/12/1
Single-cell gel electrophoresis (SCGE), or comet assay, has the ability to detect damage at the single cell level and has not been reported for equine sperm. The ability to detect nuclear damage at ...