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利用ISSR-PCR方法分析单叶蔓荆居群的遗传多样性
单叶蔓荆 ISSR-PCR 遗传多样性
2012/8/16
对山东和江西单叶蔓荆的4个居群80个样本进行资源考察和种内遗传变异情况分析。 方法 :利用ISSR分子标记技术。 结果 :从100条ISSR引物中筛选出15条特异性强、稳定性好的引物进行ISSR分析。共获得129个位点,其中多态位点数115个,多态位点百分率为89.15%。利用PopGene软件进行分析,结果表明居群之间的平均多态位点百分率为71.89%,Shonnon表型多样性指数(I)平均值为...
Simple and rapid identification of most medically important Candida species by a PCR-restriction enzyme method
Candida PCR-restriction enzyme
2009/12/24
Opportunistic fungal infections including candidiasis have increased dramatically in recent years. Most medically important fungi are Candida species. Rapid identification od Candida isolates to the s...
Screening of prognostic factors using multiplex RT-PCR technique on different leukemic cell lines
Multiplex RT-PCR T-ALL oncogene leukemia cell
2009/12/18
Background: Leukemia is one of the most common pediatric malignancies. T-cell Acute Lymphoblastic Leukemia (T-ALL) accounts for 15% of hematopoetic cancers. It has been well understood that identifica...
Application of PCR-RFLP to Rapid Identification of the Main Pathogenic Dermatophytes from Clinical Specimens
Dermatophytosis PCR-RFLP ITS region Identification Dermatophyte species
2009/12/18
Background: In the present study, a PCR-RFLP based molecular technique was designed to rapid identification of dermatophytes in clinical specimens. Skin scrapings obtained from human cases suspec...
Association between Endothelial Selectin (E-Selectin) Gene Polymorphisms and E-Selectin Level with Visceral Leishmaniais, in an ARMS-PCR Based Study
Soluble E-selectin Gene Polymorphism Visceral Leishmaniasis Human ARMS-PCR Ser128Arg 554Leu/Phe
2009/12/18
Background: In the visceral leishmaniasis (VL), parasites reside in reticuluendothelial system, mainly in macrophages. Endothelial Selectin (E-selectin) might play an important role in leukocyte-endot...
Detection of Brucella by Peripheral Blood PCR and Comparison with Culture and Serological Methods in Suspected Cases
Brucella Culture and serological methods PCR
2009/12/17
Background: Brucellosis is a zoonotic disease and its symptoms and appearances are not exclusive in human and its traditional diagnosis is based on blood culture and serological methods. For more...
Detection of Leishmania major In Naturally Infected Sand Flies Using Semi Nested-PCR
Leishmania major Sand fly Leishmaniasis PCR Iran
2009/12/17
Background: The aim of this study was to assess Leishmania infection in sand fly species from areas where leishmaniasis is endemic. This is important for prediction of the risk and expansion of the di...
Determination of HBV Genotypes among Hbs Ag Positive Blood Donors in Tehran,Iran Using PCR-RFLP
HBV Hbs Ag PCR-RFLP Blood Donors Iran
2009/12/17
Background: Hepatitis B virus (HBV) is one of the major causative agents of acute and chronic liver disease worldwide and is believed to be responsible for a million deaths annually. On the basis of a...
Single Strand Conformation Polymorphism analysis of PCR-amplified rDNA to differentiate medically important Aspergillus Species
SSCP Aspergillus Identification PCR
2009/12/9
Background: Aspergillus species are associated with allergic bronchopulmonary disease, mycotic keratitis, otomycosis, nasal sinusitis and invasive infection. In this study, we developed a PCR-Sin...
Clinical Features of Cutaneous Leishmaniasis and Direct PCR-Based Identification of Parasite Species in A New Focus in Southeast of Iran
Cutaneous leishmaniasis Leishmania major Iran
2009/12/7
Background: To clinically characterize the cutaneous leishmaniasis and identify the causative parasite species in Mirjaveh, an important geographical region across the border of Iran-Pakistan at South...
Investigation of p53 codon72 polymorphism in oral squamous cell carcinoma (SCC) specimens and normal population by PCR
Squamous cell carcinoma Mouth neoplasm Codon Genotype Exon Polymorphism p53 Polymerase chain reaction
2009/12/7
Background and Aim: A single nucleotide polymorphism at codon 72 of the p53 gene alters the p53 protein structure and affects its activity. This polymorphism depends on geographic regions and race. Al...
Comparison of Integrated Cell Culture -RT-PCR & Cell Culture Methods for Detection of Enteroviruses
Enteroviruses Sewage ICC-RT-PCR Iran
2009/11/26
Background: Generally, sewage exposed water could be potentially contaminated with enteroviruses. For this reason, enterovirus isolation from sewage specimens is one of the most sensitive indicat...
目的：探讨用重组PCR技术对人单核细胞趋化蛋白-1（hμMCP-1）基因cDNA进行缺失突变，构建N末端缺失7个氨基酸的编码序列hμMCP-1突变体-hμMCP-1（7ND）cDNA，以期实现7ND基因治疗抑制MCP-1活性。 方法： 根据缺失前后的两段基因片段A和B分别设计两对引物即内引物与外引物，第一轮PCR反应通过每段各自的内外引物，分别获得加有互补末端的A+和B+DNA片段。然后进行第二轮...
HIV-1逆转录过程及应用PCR检测
HIV-1 逆转录 聚合酶链反应
2009/11/25
人类免疫缺陷病毒(human immunodeficiency virus，HIV)作为逆转录病毒，在进入宿主细胞以后其正链RNA基因组就在逆转录酶的作用下开始逆转录合成双链的DNA。HIV-1逆转录的过程大致包括以下几个步骤：负链强力终止DNA的起始合成，负链强力终止DNA的链转移，正链DNA的合成起始，正链强力终止DNA的链转移和最终完整双链DNA的合成。逆转录是HIV-1复制的重要环节，现已...