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Purification and evaluation of somatic, excretory-secretory and Cysteine proteinase antigens of Fasciola Hepatica using IgG-ELISA in diagnosing Fascioliasis
Purification evaluation of somatic excretory-secretory Cysteine proteinase antigens of Fasciola Hepatica IgG-ELISA diagnosing Fascioliasis
2010/1/5
Fasciolosis, or liver fluke disease, caused by parasites of the genus Fasciola is emerging as an important disease in man and animals, in the world and Iran, particularly in nortern parts. The economi...
间接免疫荧光试验检测登革IgG的应用
登革热 间接免疫荧光试验 IgG抗体
2009/11/11
1995年广东省番禺等市发生登革热流行，应用间接免疫荧光试验检测58份待测登革IgG的标本,38份阳性，阳性率65.52％(38/58)；检测疑似登革热患者早期血标本51份，用C6/36细胞分离出28株登革I型病毒，阳性率54.90％(28/51)，证实本次流行是登革I型病毒所引起，二者检出率差异无显著性(X2=1.28，P>0.05)。IgG最早检出于发病后第三天，一周后达...
目的： 建立一种简便快捷的分离纯化肾病患者尿IgG亚类的方法。 方法： 收集的肾病患者24h尿液经硫酸铵盐析、DEAE-cellulose 离子交换层析和葡萄球菌A蛋白（SPA ）亲和层析等3步分离纯化，即可得到IgG1、IgG2、IgG3、IgG4 4种亚类，最后用Western blotting和SDS－PAGE 电泳的方法鉴定样品及其纯度。 结果： SPA亲和层析洗脱曲线显示有4个独立的洗脱...
IgG Subclass Levels in ELF in COPD
Bronchoalveolar lavage (BAL) epithelial lining fluid (ELF) chronic obstructive pulmonary disease (COPD) immunoglobulin deficiency immunoglobulin subclass deficiency
2009/6/29
Objective: Deficiency of immunoglobulin (Ig) may be responsible for recurrent infections in chronic obstructive pulmonary disease (COPD). Our aim was to investigate the level of IgG subclass (IgGsc) i...