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Synchronous assay for human sperm capacitation and the acrosome reaction
human sperm capacitation acrosome reaction
2009/2/23
A synchronous acrosome reaction system was established for human spermatozoa. Seminal plasma is removed from the spermatozoa by centrifugation and the washed spermatozoa are capacitated in a modif...
Evidence for a role of cyclooxygenase（prostaglandin synthetase）and prostaglandins in the sperm acrosome reaction and fertilization
spermatozoa fertilization acrosome reaction cyclooxygenase prostaglandin inhibitor
2009/2/23
Three cyclooxygenase (prostaglandin synthetase) inhibitors, indomethacin, phenylbutazone, and oxyphenbutazone, decreased fertilization in vitro when mixed with capacitated mouse spermatozoa before a...
A study of phospholipase in albumin and its role in inducing the acrosome reaction of guinea pig spermatozoa in vitro
capacitation acrosome reaction spermatozoa
2009/2/19
Phospholipase activity associated with guinea pig spermatozoa during capacitation and the acrosome reaction (AR) was determined by measuring the production of [14C] glycerylphosphorylcholine (GPC) f...
The Effect of Inhibitors of Trypsin and Phospholipase A2 on the Penetration of Zona Pellucida-free Hamster Eggs by Acrosome-reacted Hamster Sperm
Trypsin Phospholipase A2 Penetration Zona Pellucida-free Hamster Eggs
2009/2/19
The involvement of a trypsin-like enzyme and a phospholipase A2 in hamster sperm-egg fusion was investigated. Previously acrosome-reacted sperm and zona pellucida-free eggs were incubated in a low...
The Effect of ATP Depletion upon the Acrosome Reaction in Guinea Pig Sperm
sperm motility ATP depletion acrosome
2009/2/19
The suspension of guinea pig spermatozoa in substrate-free medium results in the cessation of sperm motility and a 94% reduction in adenosine triphosphate (ATP) levels within minutes. ATP depletion wa...
Acrosome reaction in dog sperm is induced by a membrane-localized progesterone receptor
Canine spermatozoa prostatic fluid
2009/2/18
The aim of this study was to investigate whether the dog sperm acrosome reaction can be induced by progesterone and whether the action of progesterone is mediated by binding of progesterone to a r...
The human acrosome reaction is highly sensitive to inhibition by cyclodiene insecticides
Sperm fertilization chiordane endosulfan
2009/2/17
The mammalian sperm acrosome reaction (AR) is essential to fertilization. It can be initiated in vitro by progesterone, a putative physiological initiator that helps to activate sperm GABA(A) recept...
Time course of spontaneous in vitro sperm acrosome reaction
Capacitation fertility potential
2009/2/17
The acrosome reaction (AR) is an exocytotic process essential for sperm penetration of the zona pellucida and binding to the oocyte (DeJonge, 1994). Evaluation of in vitro AR can suggest fertility p...
Hyaluronic acid enhances the zona pellucida-induced acrosome reaction of macaque sperm
Macaca fascicularis semen capacitation fertilization
2009/2/17
Hyaluronic acid (HA) not only surrounds the zona pellucida as part of the cumulus matrix but also is present throughout the zona pellucida and the perivitelline space of many mammalian oocytes. Ho...
Analysis of the effect of nitric oxide synthase inhibition on mouse sperm employing a modified staining method for assessment of the acrosome reaction
Nitric oxide synthase acrosome reaction
2009/2/17
A commercially available staining kit (Spermac) was combined with swelling in a hypoosmotic medium (HOS) for simultaneous assessment of viability and acrosome reaction in mouse spermatozoa. We com...
Use of peanut agglutinin to assess the acrosomal status and the zona pellucida-induced acrosome reaction in stallion spermatozoa
FITC-PNA hemizona acrosome reaction
2009/2/17
Peanut agglutinin (PNA) was used to assess the sperm acrosomal status and the acrosome reaction during gamete interaction in the equine species. PNA exclusively binds to the outer acrosomal membra...
Maturation of monkey spermatozoa in the epididymis with respect to their ability to undergo the acrosome reaction
Capacitation primate development epididymis macaque
2009/2/16
Spermatozoa obtained along the length of the epididymides from five macaque monkeys (Macaca fascicularis) were examined for their ability to undergo the acrosome reaction under basal conditions and ...
Chloride efflux during the progesterone-initiated human sperm acrosome reaction is inhibited by lavendustin A, a tyrosine kinase inhibitor
GABAA receptor Cl- channel
2009/2/16
Previous studies showed that progesterone (P) can initiate the mammalian sperm acrosome reaction (AR) in vitro and that a sperm GABAA-like receptor/Cl- channel is involved in an essential Cl- effl...
Effects of glucose and other energy substrates on the hyperactivated motility of macaque sperm and the zona pellucida-induced acrosome reaction
glucose hyperactivated motility macaque sperm
2009/2/13
Energy sources in sperm capacitation media have various effects on mammalian sperm and are required for stimulation of hyperactivated motility and/or acrosome reactions in some species. The presen...
Importance of bicarbonate to the progesterone-initiated human sperm acrosome reaction
Anion channel bicarbonate sperm acrosome reaction
2009/2/13
Progesterone, a putative in vivo initiator of the human sperm acrosome reaction (AR), has previously been shown to act at the sperm plasma membrane to initiate the AR in vitro. Here, we have investi...