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A Comparison of Blood Chemistry, Reproductive Hormones, and the Development of Antisperm Antibodies After Vasectomy in Men
vasectomy antibody response testosterone luteinizing hormone
2009/2/18
Serum chemistry parameters as well as levels of follicle-stimulating hormone (FSH), luteinizing hormone (LH), testosterone, and spermimmobilizing and sperm-agglutinating antibodies were measured pri...
A panel of monoclonal antibodies against human sperm
Antisperm antibodies sperm antigens serum immunolocalization
2009/2/17
To help identify and characterize antigens involved in sperm functions and immune infertility, monoclonal antibodies (mAbs) were raised against human sperm antigens. The immunoglobulin G (IgG) fra...
Anti-bull sperm monoclonal antibodies：II. Binding changes during capacitation and influence on sperm-zona interactions in vitro
Bull sperm sperm-zona binding capacitation
2009/2/16
Anti-bull sperm monoclonal antibodies (mAbs), generated against intra-acrosomal and surface antigens, were evaluated for their functional significance. In experiment I, the influence of mAbs on the bo...
Anti-bull sperm monoclonal antibodies：I. Identification of major antigenic domains of bull sperm and manifestation of interspecies cross-reactivity
Anti-sperm antibodies antigen localization conserved
2009/2/16
The overall objective of this series of experiments is to generate immunological markers that may elucidate bull sperm surface changes in vitro. Here we report the initial experiments of the study, ...
Hexokinase present in human sperm is not tyrosine phosphorylated but its antibodies affect fertilizing capacity
Hexokinase human sperm fertilization infertility
2009/2/16
The present study was conducted to investigate the presence of hexokinase in human sperm cell, its subcellular localization, its modulation and role in capacitation/acrosome reaction, and tyrosine...
Temporal appearance of antisperm antibodies during sexual maturation of rats after obstruction of the vas deferens
Autoantibodies spermatozoa vas deferens sparmatic
2009/2/13
The appearance of serum antisperm antibodies was studied during the maturation of rats in which the vas deferens was obstructed prepubertally. Vasa deferentia were ligated and divided bilaterally in...
Detection and quantitation of sperm-bound antibodies by flow cytometry of human semen
Spermatozoa antibody infertility
2009/2/10
Detection of sperm surface antibodies is important for the diagnosis and treatment of infertility. The authors have investigated the methodologic aspects of flow cytometry (FCM) to detect sperm-bo...
Use of neonatal tolerization and chemical immunosuppression for the production of monoclonal antibodies to maturation-specific sperm surface molecules
neonatal tolerization chemical immunosuppression monoclonal antibodies
2009/2/7
Mammalian sperm acquire functional maturity as they move from the caput to the cauda epididymidis. Changes occur in the protein/glycoprotein composition of the sperm plasma membrane during this time...
Effect of sperm-associated antibodies on the acrosomal status of human sperm
Antisperm antibodies acrosome reaction infertility spermatozoa
2009/2/6
The acrosomal status of human sperm was assessed by the specific binding of Pisum sativum lectin to the acrosomal matrix. Immunoglobulin G (IgG) fractions of plasmas that were positive for IgG antis...
Absence of antisperm antibodies in anejaculatory men
Agglutinating antibodies immobilizing antibodies immunobead assay
2009/2/6
Antisperm antibodies were assessed in the serum samples of 73 men unable to ejaculate naturally and on the sperm cells of 13 of these men. None of the serum samples were found to be positive by sp...
Preparation and characterization of monoclonal antibodies against sperm-specific lactate dehydrogenase C4
LDH-C4 monoclonal antibodies specificity
2009/2/6
LDH-C4, the testis-specific isozymes of lactate dehydrogenase (LDH), is the predominant LDH isozyme in mammalian spermatozoa. Nine monoclonal antibodies against mouse LDH-C4 have been developed. The...
Solid-phase indirect immunogold localization of human sperm antigens reacting with antisperm antibodies in human sera
immunogold light microscopy localization
2009/2/6
A simple solid-phase indirect immunogold assay for light microscopic localization of the binding of sperm-reactive antibodies in human sera to surface-associated antigenic sites on human sperm is de...
Late Hormonal Levels, Semen Parameters, and Presence of Antisperm Antibodies in Patients Treated for Testicular Torsion
Infertility function testis
2008/12/24
In spite of prompt diagnosis and either orchiectomy or preservation of the affected testis, infertility remains a significant sequel to testicular torsion. The objective of this study was to e...
Monoclonal Antibodies to Epididymis-specific Proteins Using Mice Rendered Immune Tolerant to Testicular Proteins
Neonatal tolerization epididymis monoclonal antibodies
2008/12/19
Monoclonal antibodies (mabs) have been used as a powerful tool for identification of newer sperm proteins. However, conventional hybridoma technology rarely provides chance to obtain mabs to epididy...